Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis

摘要

Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.